Pdf the fungal genus curvularia includes numerous plant. Curvularia can be easily distinguished from bipolaris and drechslera spp. Curvularia is a hyphomycete found in plants and soil of tropical and temperate countries. Curvularia leaf spot occurs in areas that experience prolonged leaf wetness for several consecutive days. The disease transmission was evaluated from seed sowing, in which after 40 days typical symptoms of curvularia sp. Curvularia leaf spot disease of foliage andor roots. Dematiaceous hyphomycete producing sympodial, pale brown, cylindrical or slightly curved phragmoconidia, with one of the cent. Novel curvularia species from clinical specimens ncbi.
The taxonomy of this complex is confusing as frequent nomenclatural changes and refinements have occurred. Treatment with a combination of topical antibiotics polymyxin b, sulfatetrimetoprim, and 10% sulfacetamide sodium, 1% miconazole, autologous serum, and 5% hypertonic saline was instituted. Curvulariafavorable response to oral itraconazole therapy in. Cochliobolus lunatus is a fungal plant pathogen that can cause disease in humans and other animals. These are curvularia lunata, curvularia pallescens and curvularia geniculata curvularia lunata. A phylogenetic and taxonomic reevaluation of the bipolaris. Individual leaves initially show yellow and green dapple patterns that extend downwards from the leaf tip. It is associated with open globe injuries and is a common cause of secondary fungal keratitis after diagnostic aspiration.
They usually have a curved appearance, having broad points at either end. Stereoscopic images of various bermudagrass leaf symptomatology. Evaluation of curvularia lunata as an biological control agent in major weeds. Among eye infections, the involvement of curvularia spp. The genus curvularia contains about 80 species, which are mostly soil or plant pathogens. A phylogenetic analysis of the genera bipolaris and curvularia has resulted in a realignment of. Curvularia lunata is a widespread contaminant of seed crops. Natural habitats plant saprobe and pathogen to cereal plants soil suitable substrates in the indoor environment paper wood products water activity unknown mode of dissemination wind. Allergic bronchopulmonary disease caused by curvularia has been reported 2. Most kinds of mold grow and spread quickly, but according to the university of adelaide website, this particular strain of mold grows especially fast.
Exo and endoglucanase production by curvularia affinis. Nigeria is identified as curvularia pallescens and its pathogenicity to maize is demonstrated by inocula tion. Comparative analysis of phylogenetic relationships. Novel curvularia species from clinical specimens article pdf available in persoonia molecular phylogeny and evolution of fungi in press1 july 2014 with 3,408 reads how we measure reads. I here present two cases, a healthy man with locally invasive, mulicentric paranasal fungal sinusitis, and a case of progressive verrucal distal onychomycosis that. Clinical importance of the genus curvularia 159 india, karnataka october 1985september 1988 67 2 2. Most commonly found on low cut turf grasses like golf and bowling greens. Mabadeje school of biological sciences, university of lagos, nigeria with plate 18 the causal agent of a leaf spot of maize in s. The teleomorphic state of the type species curvularia lunata is cochliobolus lunatus fam. We agree with the view of authors like alcorn 1983b, sivanesan. Curvulariafavorable response to oral itraconazole therapy.
Curvularia species are ubiquitous and occasionally lead to infections in humans. Isolation and molecular identification of curvularia lunata. Curvularia inaequalis curvinoverview eppo global database. Genome sequencing of the pathogen will provide important information for globally understanding its virulence mechanism. Curvularia species are important phytopathogens reported worldwide. Curvularia lunata poses an inhalation and a deep skin dermal inoculation health risk to persons with week immune system. The anamorph of this fungus is known as curvularia lunata, while cochliobolus lunatus denotes the teleomorph or sexual state. Curvularia is a hyphomycete fungus which is a facultative pathogen of many plant species.
Reports of human disease caused by this organism are rare but include. We do not use these to store personal information about you. In the current study, a sensitive polymerase chain reaction assay was developed for the detection of c. Novel curvularia species from clinical specimens article pdf available in persoonia molecular phylogeny and evolution of fungi in press1 july 2014 with. The ctnsensus of opinion seems to be that curvularia will invade turf that is already weakened and become a secondary pathogen. Curvularia is a species of mold that relate mostly to subtropical and tropical plant parasites however, three ubiquitous species have been recovered from human infections, principally from cases of mycotic keratitis. Curvularia definition of curvularia by medical dictionary. Curvularia pallescens boedijn hyphomycetes is redescribed with the aid of a scanning electron microscope, and the optimal cultural conditions for growing this fungus are discussed. Effects of temperature on mycelial growth sporulation and spore germination of curvularia spp. Rinaldi mg, phillips p, schwartz jg, winn re, holt gr, shagets fw, elrod j, nishioka g, aufdemorte tb. Most curvularia are found in tropical regions, though a few are found in temperate zones. Curvularia is a saprobic dematiaceous mold that has been associated with a wide spectrum of human infection 1, 2. Pawar department of plant pathology, college of agriculture, dr.
Two specific forward clgd1clgd2 and one reverse primers clgd3 were designed. Curvularia lunata is a saprobic dematiaceous mould that resides primarily in soil ellis, 1966. Isolation and molecular identification of curvularia. Find related pest control products, articles and questions on curvularia spp. Three isolates of curvularia were obtained from diseased eucalyptus sp. Rapid and sensitive detection of curvularia lunata.
Curvularia diseases produce irregularly shaped patches of thinned turf, often coalescing to affect larger areas. I here present two cases, a healthy man with locally invasive, mulicentric paranasal fungal sinusitis, and a case of progressive verrucal. In vitro evaluation of fungicides and organics against. The objectives are to isolate and characterize curvularia and related species from leaf spot of rice by using morphological and molecular characterization and to determine the phylogenetic relationship between the isolated fungi.
Proceedings of the association for plant protection of hokuriku, no. Pdf clinical importance of the genus curvularia researchgate. Studies on the discoloration of rice kernels caused by fungi. Aug 25, 2012 three genera, cochliobolus, bipolaris and curvularia form a complex that contains many plant pathogens, mostly on grasses poaceae with a worldwide distribution. Pdf novel curvularia species from clinical specimens. Morphological and molecular characterization of curvularia lunata. Culture of the lesion found heavy growth of enterococcus faecalis and curvularia spp. Aug 09, 20 curvularia is a hyphomycete found in plants and soil of tropical and temperate countries.
Soil compaction and excessive levels of nitrogen and thatch. General information about curvularia inaequalis curvin this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. The spores of curvularia occur individually, with three or more transverse divisions, or septa. Wintergrass, kentucky bluegrass, bentgrass, fescue, couch. Exo and endoglucanase production by curvularia affinis using. Three genera, cochliobolus, bipolaris and curvularia form a complex that contains many plant pathogens, mostly on grasses poaceae with a worldwide distribution. We report the genome sequences of a highly virulent c. Cytological analysis and nuclear condition, observed through the hclgiemsa technique, showed vegetative and reproductive structures hypha and conidia formed by uni. There is no clear morphological boundary between the asexual genera bipolaris and curvularia, and some species show. Example of common leaf tip necrosis of cut leaves 24 to 48 h after inoculation with bipolaris spp. In vitro evaluation of fungicides and organics against curvularia lunata and curvularia pallescens causing leaf blight in gladiolus d. Natural habitats plant saprobe and pathogen to cereal plants soil suitable substrates in the indoor environment paper wood products water activity unknown mode of dissemination wind allergenic potential type i asthma and hay fever a relatively common cause of. Evaluation of curvularia lunata as an biological control.
Curvularia has a downy or suedelike texture and may be a medium shade of brown to dark brown or nearly black in color. Evaluation of pathogenicity of bipolaris and curvularia. Evaluation of pathogenicity of bipolaris and curvularia spp. In immunosuppressed patients, infections are often serious, and systemic dissemination is not uncommon. Learn how to control curvularia spp with these easy to use products. Based on these morphological characters, the fungus was identified as curvularia spicifera bainier boedijn.
Identification of curvularia lunata by polymerase chain. Individual leaves initially show yellow and green dappled patterns that extend downwards from the leaf tip. Recent studies have shown that morphological identification does not correlate with molecular identification manamgoda et al. Curvularia is a mold which is associated with allergy in the literature and frequently reported in mold spore counts. In many countries, agricultural residues are generated in large quantities, and most of these are underutilized and considered waste, especially in developing countries. Cochliobolus lunatus is the most commonly reported species in clinical cases of reported. Curvularia is a hyphomycete associated with over 60 host genera manamgoda et al. Find, read and cite all the research you need on researchgate. Continuing to use this website means you agree to our use of cookies. There is no clear morphological boundary between the asexual genera bipolaris and curvularia. Curvularia leaf spot disease of foliage andor roots greencast. In this study, curvularia affinis was isolated from the leaves of phaseolus vulgaris l.
Nigeria is identified as curvularia pallescens and its pathogenicity to maize is demonstrated by inocula tion experiments. After 8 days of medical therapy, an improvement of clinical signs. The shape of the spores is what is so unique to curvularia, and in fact, is where we get the name. Most kinds of mold grow and spread quickly, but according to the university of adelaide website, this particular strain of. To date, however, only five known reports of endophthalmitis caused by curvularia spp.
Curvularia lunata wakker boed, the causative agent of curvularia leaf spot in maize, was determined according to conidiophore and conidium morphology in a previous study. Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in china. India among the various flowering plants that provides. However, applicability of this region to discern curvularia spp. The isolates were analyzed in terms of phylogenetic relationships, morphology, and pathogenicity. A phylogenetic analysis of the genera bipolaris and curvularia has resulted. Morphological and molecular characterization of curvularia. Cryptic species of curvularia in the culture collection of. The fungus is an excellent saprophyte, meaning it exists or persists in dead and dying tissue. The purified amplicons were read in both directions using original pcr. Fungal isolation was done from diseased rice leaves showing leaf spot symptoms collected throughout peninsular malaysia. Cytological analysis and nuclear condition, observed through the hclgiemsa technique, showed vegetative and reproductive structures hypha and conidia formed by. Curvularia may be found in almost any grass debris, even if the turf is healthy.
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